Composite

Part:BBa_K2789012

Designed by: Yifan Song   Group: iGEM18_WHU-China   (2018-10-05)


In the absence of ligand, the expression of toxin inhibits the reproduction of bacteria so as to pre

ParD is a toxin coding gene that encodes toxins that express toxins that inhibit DNA helicase activity, thereby inhibiting bacterial reproduction. ParE is an antitoxin coding gene that encodes and parD encodes the antitoxin proteins that bind to each other to render them toxic, but the antitoxin proteins are unstable and easily degraded by serine protease. Antitoxins can prevent the effects of endogenous expression of toxins, but in the case of large amounts of toxin expression, antitoxins are difficult to antagonise toxins, which can lead to the death of bacteria Used to prevent horizontal gene transfer of bacteria


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 242
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None