DNA

Part:BBa_K2785013

Designed by: Zemeng Wei   Group: iGEM18_Pittsburgh   (2018-10-09)


nCas9 under Xylose Promoter

Cas9 protein without one endonuclease domain. This is improved from Part:BBa_K1323002. nCas9 can be a part of CRISPR/Cas9 base editor fusion.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1476
    Illegal BamHI site found at 4894
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None