Coding

Part:BBa_K2785012

Designed by: Zemeng Wei   Group: iGEM18_Pittsburgh   (2018-10-09)


Cytidine Deaminase (CDA)

For our project it is a key component of our CRISPR/Cas9 base editor fusion protein, this enzyme is directly responsible for single nucleotide mutation made in the DNA. Once gRNA directs the modified Cas9 protein (either dCas9 or nCas9) to the correct location in the DNA, the cytidine deaminase removes an amide group (-NH2) from cytidine thereby producing uridine. The uridine, through natural cell repair, is ultimately converted to a thymidine [C->T]. Essentially, we are just using the Cas9 protein as a shuttle for our CDA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 694
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None