DNA

Part:BBa_K2785009

Designed by: Zemeng Wei   Group: iGEM18_Pittsburgh   (2018-10-09)


DOMINO gRNA

This construct consists of two gRNAs controlled by separate inducible promoters. gRNA #1 directs the base editor to make a mutation on the recording plasmid at the current frame. Once that mutation is made, this allows gRNA #1 to recognize the next frame. By constantly shifting the frame that is able to record, the system is keeping track of elapsed time. Under the presence of a stimulus, gRNA #2 is produced and directs the base editor to mark the current frame with a unique mutation.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 579
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None