Part:BBa_K277127

3L.3_23.D3.06

3L.3_23.D3.06 is 779 bases long and is cloned into the pGem-T vector.

3L.3_23.D3.06 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. The whole synthetic chromosome may be viewed at http://macbeth.clark.jhu.edu/cgi-bin/gbrowse 3L.3_23.D3.06 is a constituent of 3L.3_23.D3 (along with 3L.3_23.D3.01, 3L.3_23.D3.02, 3L.3_23.D3.03, 3L.3_23.D3.04, 3L.3_23.D3.05, 3L.3_23.D3.07, 3L.3_23.D3.08, 3L.3_23.D3.09, 3L.3_23.D3.10, 3L.3_23.D3.11, and 3L.3_23.D3.12.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

gene YCL001W (562..+1128) Protein involved in retention of membrane proteins%2C including Sec12p%2C in the ER%3B localized to Golgi%3B functions as a retrieval receptor in returning membrane proteins to the ER

reverse_primer YCL002C_tagr1v1 (122..149)

gene YCL002C (-433..322) Putative protein of unknown function%3B YCL002C is not an essential gene

forward_primer YCL001W_tagf1v1 (672..699)

Sequence (corresponds to coordinates 92201..92979 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 302
  Illegal PstI site found at 450
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 302
  Illegal PstI site found at 450
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 302
  Illegal PstI site found at 450
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 302
  Illegal PstI site found at 450
  
• 1000
  COMPATIBLE WITH RFC[1000]