Part:BBa_K277125

3L.3_23.D3.04

3L.3_23.D3.04 is 748 bases long and is cloned into the pGem-T vector.

3L.3_23.D3.04 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. The whole synthetic chromosome may be viewed at http://macbeth.clark.jhu.edu/cgi-bin/gbrowse 3L.3_23.D3.04 is a constituent of 3L.3_23.D3 (along with 3L.3_23.D3.01, 3L.3_23.D3.02, 3L.3_23.D3.03, 3L.3_23.D3.05, 3L.3_23.D3.06, 3L.3_23.D3.07, 3L.3_23.D3.08, 3L.3_23.D3.09, 3L.3_23.D3.10, 3L.3_23.D3.11, and 3L.3_23.D3.12.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

gene YCL004W (-698..+867) Phosphatidylglycerolphosphate synthase%2C catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis

reverse_primer YCL004W_tagr2v1 (144..171)

Sequence (corresponds to coordinates 90728..91475 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 311
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 311
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 311
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 311
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 718