Part:BBa_K277118

3L.3_23.D2.14

3L.3_23.D2.14 is 751 bases long and is cloned into the pGem-T vector.

3L.3_23.D2.14 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. The whole synthetic chromosome may be viewed at http://macbeth.clark.jhu.edu/cgi-bin/gbrowse 3L.3_23.D2.14 is a constituent of 3L.3_23.D2 (along with 3L.3_23.D2.01, 3L.3_23.D2.02, 3L.3_23.D2.03, 3L.3_23.D2.04, 3L.3_23.D2.05, 3L.3_23.D2.06, 3L.3_23.D2.07, 3L.3_23.D2.08, 3L.3_23.D2.09, 3L.3_23.D2.10, 3L.3_23.D2.11, 3L.3_23.D2.12, 3L.3_23.D2.13, 3L.3_23.D2.15, 3L.3_23.D2.16, and 3L.3_23.D2.17.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

gene YCL009C (-279..650) Regulatory subunit of acetolactate synthase%2C which catalyzes the first step of branched-chain amino acid biosynthesis%3B enhances activity of the Ilv2p catalytic subunit%2C localizes to mitochondria

reverse_primer YCL009C_tagr1v1 (138..165)

Sequence (corresponds to coordinates 85928..86678 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]