Part:BBa_K277110
3L.3_23.D2.06
3L.3_23.D2.06 is 744 bases long and is cloned into the pGem-T vector.
3L.3_23.D2.06 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. The whole synthetic chromosome may be viewed at http://macbeth.clark.jhu.edu/cgi-bin/gbrowse 3L.3_23.D2.06 is a constituent of 3L.3_23.D2 (along with 3L.3_23.D2.01, 3L.3_23.D2.02, 3L.3_23.D2.03, 3L.3_23.D2.04, 3L.3_23.D2.05, 3L.3_23.D2.07, 3L.3_23.D2.08, 3L.3_23.D2.09, 3L.3_23.D2.10, 3L.3_23.D2.11, 3L.3_23.D2.12, 3L.3_23.D2.13, 3L.3_23.D2.14, 3L.3_23.D2.15, 3L.3_23.D2.16, and 3L.3_23.D2.17.)
This part contains at least part of the following features (positions offset from first base of sequence):
kind and name offset notes
forward_primer YCL014W_tagf4v1 (719..+746)
reverse_primer YCL014W_tagr1v1 (341..368)
gene YCL014W (-2721..+2189) Protein involved in bud-site selection and required for axial budding pattern%3B localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding
Sequence (corresponds to coordinates 79929..80672 in synthetic chromosome yeast_chr3_3_23)
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 66
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 66
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 66
Illegal XhoI site found at 608 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 66
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 66
- 1000COMPATIBLE WITH RFC[1000]
| None |