Signalling

Part:BBa_K2771044

Designed by: Fabio Nunes de Mello   Group: iGEM18_USP-Brazil   (2018-10-12)


Tra inducible Synthase

Quorum sensing is a bacterial communication system that can be very useful for bacterial communication in synthetic biology. In this system, AHL (N-Acyl homoserine lactone) signaling molecules generated in a bacteria binds to a receiver in other bacteria to activate downstream gene expression. The AHL molecules are generated by a synthase and received by other bacteria through a reporter.

Among all the QS pathways, the Tra pathway is based on the production of the OC8-HSL (homoserine lactone) produced by the Tral protein. This part contains the Lasl transcribed by a promoter inducible by L-arabinose, permitting for controlled expression of the HSL signal.

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1452
    Illegal NgoMIV site found at 1779
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961

Reeference


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Categories
Parameters
None