Composite
LasSR

Part:BBa_K2771031

Designed by: Fabio Nunes de Mello   Group: iGEM18_USP-Brazil   (2018-10-12)


Las inducible Synthase and constitutive Receiver

This part encodes for two genes in a quorum sensing signaling pathway, intended in our project for measurement of genetic crosstalk with quorum sensing responsive promoters. One of the genes, transcribing for LasI, an Homoserine Lactone (HSL) Synthase, is transcribed by a promoter inducible by L-arabinose, permitting for controlled expression of the HSL signal. The other gene is constitutively expressed by an Andersen family promoter. If coupled to a gene with a promoter responsive to quorum sensing signals, this construct can create a response dependant on cell density and L-arabinose concentration. If there is a spatial difference in arabinose concentration, the HSL signal will diffuse in the medium and present a weaker response when farther to the signal origin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 1997
    Illegal NheI site found at 2020
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2373
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1459
    Illegal AgeI site found at 2570
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961

Reference


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