Coding

Part:BBa_K2771005

Designed by: Fabio Nunes de Mello   Group: iGEM18_USP-Brazil   (2018-10-12)


TraR receiver (E192W)

This part generates the A. Tumefaciens TraR receiver, which binds to the OC8 HLA and activates the pTra promoter (BBa_K2771002). The sequence has been codon optimized for expression in E. coli and differs from BBa_K2771007 by a E192W mutation that improves its activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 67
    Illegal AgeI site found at 103
    Illegal AgeI site found at 402
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference


[edit]
Categories
Parameters
None