Part:BBa_K277089

3L.3_23.C3.10

3L.3_23.C3.10 is 749 bases long and is cloned into the pGem-T vector.

3L.3_23.C3.10 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. The whole synthetic chromosome may be viewed at http://macbeth.clark.jhu.edu/cgi-bin/gbrowse 3L.3_23.C3.10 is a constituent of 3L.3_23.C3 (along with 3L.3_23.C3.01, 3L.3_23.C3.02, 3L.3_23.C3.03, 3L.3_23.C3.04, 3L.3_23.C3.05, 3L.3_23.C3.06, 3L.3_23.C3.07, 3L.3_23.C3.08, 3L.3_23.C3.09, 3L.3_23.C3.11, 3L.3_23.C3.12, 3L.3_23.C3.13, and 3L.3_23.C3.14.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

ARS ARS306 (229..448) Autonomously Replicating Sequence on Chromosome III

forward_primer YCL026C-A_tagf1v1 (656..683)

loxP_site loxPsym_YCL026C-A (472..505)

gene YCL026C-A (509..+1090) Protein of unknown function%2C involved in the integration of lipid signaling pathways with cellular homeostasis

Sequence (corresponds to coordinates 64943..65691 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 448
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 713
  
• 1000
  COMPATIBLE WITH RFC[1000]