Part:BBa_K277087
3L.3_23.C3.08
3L.3_23.C3.08 is 669 bases long and is cloned into the pGem-T vector.
3L.3_23.C3.08 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. The whole synthetic chromosome may be viewed at http://macbeth.clark.jhu.edu/cgi-bin/gbrowse 3L.3_23.C3.08 is a constituent of 3L.3_23.C3 (along with 3L.3_23.C3.01, 3L.3_23.C3.02, 3L.3_23.C3.03, 3L.3_23.C3.04, 3L.3_23.C3.05, 3L.3_23.C3.06, 3L.3_23.C3.07, 3L.3_23.C3.09, 3L.3_23.C3.10, 3L.3_23.C3.11, 3L.3_23.C3.12, 3L.3_23.C3.13, and 3L.3_23.C3.14.)
This part contains at least part of the following features (positions offset from first base of sequence):
kind and name offset notes
gene YCL026C-B (658..+1239) Putative protein of unknown function%3B green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
gene YCL027W (-978..560) Membrane protein localized to the shmoo tip%2C required for cell fusion%3B expression regulated by mating pheromone%3B proposed to coordinate signaling%2C fusion%2C and polarization events required for fusion%3B potential Cdc28p substrate
loxP_site loxPsym_YCL027W (564..597)
forward_primer YCL027W_tagf2v1 (68..95)
reverse_primer YCL027W_tagr2v1 (266..293)
Sequence (corresponds to coordinates 63461..64129 in synthetic chromosome yeast_chr3_3_23)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 318
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |