Part:BBa_K277082

3L.3_23.C3.03

3L.3_23.C3.03 is 720 bases long and is cloned into the pGem-T vector.

3L.3_23.C3.03 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. The whole synthetic chromosome may be viewed at http://macbeth.clark.jhu.edu/cgi-bin/gbrowse 3L.3_23.C3.03 is a constituent of 3L.3_23.C3 (along with 3L.3_23.C3.01, 3L.3_23.C3.02, 3L.3_23.C3.04, 3L.3_23.C3.05, 3L.3_23.C3.06, 3L.3_23.C3.07, 3L.3_23.C3.08, 3L.3_23.C3.09, 3L.3_23.C3.10, 3L.3_23.C3.11, 3L.3_23.C3.12, 3L.3_23.C3.13, and 3L.3_23.C3.14.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

forward_primer YCL029C_tagf1v1 (321..348)

reverse_primer YCL029C_tagr1v1 (567..594)

gene YCL029C (-489..+833) Microtubule-associated protein%2C component of the interface between microtubules and kinetochore%2C involved in sister chromatid separation%3B essential in polyploid cells but not in haploid or diploid cells%3B ortholog of mammalian CLIP-170

Sequence (corresponds to coordinates 59734..60453 in synthetic chromosome yeast_chr3_3_23

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 591
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 531
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]