Part:BBa_K277069

3L.3_23.C1.08

3L.3_23.C1.08 is 682 bases long and is cloned into the pGem-T vector.

3L.3_23.C1.08 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. The whole synthetic chromosome may be viewed at http://macbeth.clark.jhu.edu/cgi-bin/gbrowse 3L.3_23.C1.08 is a constituent of 3L.3_23.C1 (along with 3L.3_23.C1.01, 3L.3_23.C1.02, 3L.3_23.C1.03, 3L.3_23.C1.04, 3L.3_23.C1.05, 3L.3_23.C1.06, 3L.3_23.C1.07, and 3L.3_23.C1.09.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

loxP_site loxPsym_YCL035C (542..575)

gene YCL035C (579..+911) Hydroperoxide and superoxide-radical responsive heat-stable glutathione-dependent disulfide oxidoreductase with active site cysteine pair%3B protects cells from oxidative damage

gene YCL036W (-1270..430) Protein of unknown function%2C identified as a high-copy suppressor of a dbp5 mutation

Sequence (corresponds to coordinates 50772..51453 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 362
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 362
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 362
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 362
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 362
  
• 1000
  COMPATIBLE WITH RFC[1000]