Part:BBa_K277066
3L.3_23.C1.05
3L.3_23.C1.05 is 719 bases long and is cloned into the pGem-T vector.
3L.3_23.C1.05 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. The whole synthetic chromosome may be viewed at http://macbeth.clark.jhu.edu/cgi-bin/gbrowse 3L.3_23.C1.05 is a constituent of 3L.3_23.C1 (along with 3L.3_23.C1.01, 3L.3_23.C1.02, 3L.3_23.C1.03, 3L.3_23.C1.04, 3L.3_23.C1.06, 3L.3_23.C1.07, 3L.3_23.C1.08, and 3L.3_23.C1.09.)
This part contains at least part of the following features (positions offset from first base of sequence):
kind and name offset notes
reverse_primer YCL037C_tagr1v1 (492..519)
forward_primer YCL037C_tagf1v1 (249..276)
gene YCL037C (-681..623) Cytoplasmic RNA-binding protein that associates with translating ribosomes%3B involved in heme regulation of Hap1p as a component of the HMC complex%2C also involved in the organization of actin filaments%3B contains a La motif
Sequence (corresponds to coordinates 48531..49249 in synthetic chromosome yeast_chr3_3_23)
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 278
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 273
Illegal PstI site found at 278 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 710
Illegal BamHI site found at 700 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 278
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 278
Illegal NgoMIV site found at 523 - 1000COMPATIBLE WITH RFC[1000]
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