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Part:BBa_K277064

Designed by: James DiCarlo   Group: iGEM09_Johns_Hopkins-BAG   (2009-10-21)

3L.3_23.C1.03


3L.3_23.C1.03 is 762 bases long and is cloned into the pGem-T vector.

3L.3_23.C1.03 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. The whole synthetic chromosome may be viewed at http://macbeth.clark.jhu.edu/cgi-bin/gbrowse 3L.3_23.C1.03 is a constituent of 3L.3_23.C1 (along with 3L.3_23.C1.01, 3L.3_23.C1.02, 3L.3_23.C1.04, 3L.3_23.C1.05, 3L.3_23.C1.06, 3L.3_23.C1.07, 3L.3_23.C1.08, and 3L.3_23.C1.09.)

This part contains at least part of the following features (positions offset from first base of sequence): kind and name offset notes

Sequence (corresponds to coordinates 47031..47792 in synthetic chromosome yeast_chr3_3_23)

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 539
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 539
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 539
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 539
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 539
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
n/a 3L.3_23.C1.03