Part:BBa_K277063

3L.3_23.C1.02

3L.3_23.C1.02 is 749 bases long and is cloned into the pGem-T vector.

3L.3_23.C1.02 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. The whole synthetic chromosome may be viewed at http://macbeth.clark.jhu.edu/cgi-bin/gbrowse 3L.3_23.C1.02 is a constituent of 3L.3_23.C1 (along with 3L.3_23.C1.01, 3L.3_23.C1.03, 3L.3_23.C1.04, 3L.3_23.C1.05, 3L.3_23.C1.06, 3L.3_23.C1.07, 3L.3_23.C1.08, and 3L.3_23.C1.09.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

forward_primer YCL038C_tagf2v1 (305..332)

reverse_primer YCL038C_tagr2v1 (545..572)

Sequence (corresponds to coordinates 46293..47041 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 147
  Illegal EcoRI site found at 283
  Illegal PstI site found at 521
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 147
  Illegal EcoRI site found at 283
  Illegal PstI site found at 521
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 147
  Illegal EcoRI site found at 283
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 147
  Illegal EcoRI site found at 283
  Illegal PstI site found at 521
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 147
  Illegal EcoRI site found at 283
  Illegal PstI site found at 521
  
• 1000
  COMPATIBLE WITH RFC[1000]