Part:BBa_K277061

3L.3_23.B3.09

3L.3_23.B3.09 is 861 bases long and is cloned into the pGem-T vector.

3L.3_23.B3.09 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B3.09 is a constituent of 3L.3_23.B3 (along with 3L.3_23.B3.01, 3L.3_23.B3.02, 3L.3_23.B3.03, 3L.3_23.B3.04, 3L.3_23.B3.05, 3L.3_23.B3.06, 3L.3_23.B3.07, and 3L.3_23.B3.08.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

gene YCL039W (-1686..551) Protein of unknown function%2C involved in proteasome-dependent catabolite inactivation of fructose-1%2C6-bisphosphatase%3B contains six WD40 repeats%3B computational analysis suggests that Gid7p and Moh1p have similar functions

mutation_affecting_coding_sequence YCL038C_re_remove_MmeI (843..854) removal of MmeI

loxP_site loxPsym_YCL038C (641..674)

loxP_site loxPsym_YCL039W (555..588)

Sequence (the first 861 bases correspond to coordinates 44705..45565 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 478
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 478
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 303
  Illegal BamHI site found at 68
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 478
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 478
  
• 1000
  COMPATIBLE WITH RFC[1000]