Part:BBa_K277054

3L.3_23.B3.02

3L.3_23.B3.02 is 756 bases long and is cloned into the pGem-T vector.

3L.3_23.B3.02 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B3.02 is a constituent of 3L.3_23.B3 (along with 3L.3_23.B3.01, 3L.3_23.B3.03, 3L.3_23.B3.04, 3L.3_23.B3.05, 3L.3_23.B3.06, 3L.3_23.B3.07, 3L.3_23.B3.08, and 3L.3_23.B3.09.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

gene YCL043C (-509..+1059) Protein disulfide isomerase%2C multifunctional protein resident in the endoplasmic reticulum lumen%2C essential for the formation of disulfide bonds in secretory and cell-surface proteins%2C unscrambles non-native disulfide bonds

reverse_primer YCL043C_tagr2v1 (274..301)

forward_primer YCL043C_tagf2v1 (55..82)

Sequence (the first 756 bases correspond to coordinates 39502..40257 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 151
  
• 1000
  COMPATIBLE WITH RFC[1000]