Other

Part:BBa_K277050

Designed by: James DiCarlo   Group: iGEM09_Johns_Hopkins-BAG   (2009-10-21)


3L.3_23.B2.10


3L.3_23.B2.10 is 755 bases long and is cloned into the pGem-T vector.

3L.3_23.B2.10 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B2.10 is a constituent of 3L.3_23.B2 (along with 3L.3_23.B2.01, 3L.3_23.B2.02, 3L.3_23.B2.03, 3L.3_23.B2.04, 3L.3_23.B2.05, 3L.3_23.B2.06, 3L.3_23.B2.07, 3L.3_23.B2.08, 3L.3_23.B2.09, 3L.3_23.B2.11, and 3L.3_23.B2.12.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

gene YCL045C (-1389..+893) Protein of unknown function%3B green fluorescent protein (GFP)-fusion protein localizes to the ER%3B interacts with Gal80p%3B YCL045C is not an essential gene

mutation_affecting_coding_sequence YCL045C_re_remove_BpuEI (378..386) removal of BpuEI

reverse_primer YCL045C_tagr2v1 (201..228)

gene YCL046W (628..+951) Hypothetical protein

mutation_affecting_coding_sequence YCL045C_re_remove_Bpu10I (30..38) removal of Bpu10I

Sequence (the first 755 bases correspond to coordinates 36318..37072 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 40
    Illegal XbaI site found at 217
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 740
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 40
    Illegal XbaI site found at 217
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 40
    Illegal XbaI site found at 217
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 737


[edit]
Categories
Parameters
None