Part:BBa_K277047

3L.3_23.B2.07

3L.3_23.B2.07 is 750 bases long and is cloned into the pGem-T vector.

3L.3_23.B2.07 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B2.07 is a constituent of 3L.3_23.B2 (along with 3L.3_23.B2.01, 3L.3_23.B2.02, 3L.3_23.B2.03, 3L.3_23.B2.04, 3L.3_23.B2.05, 3L.3_23.B2.06, 3L.3_23.B2.08, 3L.3_23.B2.09, 3L.3_23.B2.10, 3L.3_23.B2.11, and 3L.3_23.B2.12.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

mutation_affecting_coding_sequence YCL047C_re_remove_MmeI (429..440) removal of MmeI

mutation_affecting_coding_sequence YCL047C_re_remove_BpuEI (99..107) removal of BpuEI

forward_primer YCL047C_tagf1v1 (120..147)

reverse_primer YCL047C_tagr1v1 (342..369)

mutation_affecting_coding_sequence YCL047C_re_remove_Bpu10I (444..452) removal of Bpu10I

gene YCL047C (-99..677) Hypothetical protein

Sequence (the first 750 bases correspond to coordinates 34066..34815 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 604
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 604
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 604
  Illegal NgoMIV site found at 442
  Illegal AgeI site found at 82
  
• 1000
  COMPATIBLE WITH RFC[1000]