Part:BBa_K277036

3L.3_23.B1.10

3L.3_23.B1.10 is 678 bases long and is cloned into the

pGem-T vector.

3L.3_23.B1.10 was designed as a piece of

synthetic chromosome 3 with the goal of minimizing and

stabilizing that chromosome and to that end has had any

tRNAs, introns, repeat regions, and transposons that were

present in the wildtype chromosome removed. In addition a

very few gene sequences were slightly recoded to add or

remove restriction enzyme recognition sites to facilitate

assembly; most gene sequences were slightly recoded to

introduce unique primers for diagnostic PCR

amplification, and some gene sequences were slightly

recoded to address the distribution of stop codon usage.

3L.3_23.B1.10 is a constituent of 3L.3_23.B1 (along with

3L.3_23.B1.01, 3L.3_23.B1.02, 3L.3_23.B1.03,

3L.3_23.B1.04, 3L.3_23.B1.05, 3L.3_23.B1.06,

3L.3_23.B1.07, 3L.3_23.B1.08, 3L.3_23.B1.09,

3L.3_23.B1.11, 3L.3_23.B1.12, 3L.3_23.B1.13, and

3L.3_23.B1.14.)

This part contains at least part of the

following features (positions offset from first base of

sequence):

kind and name offset notes

gene

YCL051W (217..+1968) Protein involved in control of

cell wall structure and stress response%3B inhibits Cbk1p

protein kinase activity%3B overproduction confers

resistance to cell-wall degrading enzymes

forward_primer YCL051W_tagf2v1 (630..657)

Sequence

(the first 678 bases correspond to coordinates

25852..26529 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 353
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 315
  Illegal PstI site found at 353
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 673
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 353
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 353
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 571