Part:BBa_K277035

3L.3_23.B1.09

3L.3_23.B1.09 is 754 bases long and is cloned into the pGem-T vector.

3L.3_23.B1.09 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B1.09 is a constituent of 3L.3_23.B1 (along with 3L.3_23.B1.01, 3L.3_23.B1.02, 3L.3_23.B1.03, 3L.3_23.B1.04, 3L.3_23.B1.05, 3L.3_23.B1.06, 3L.3_23.B1.07, 3L.3_23.B1.08, 3L.3_23.B1.10, 3L.3_23.B1.11, 3L.3_23.B1.12, 3L.3_23.B1.13, and 3L.3_23.B1.14.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

reverse_primer YCL052C_tagr1v1 (213..240)

gene YCL052C (-762..488) Essential component of glycosylphosphatidylinositol-mannosyltransferase I%2C required for the autocatalytic post-translational processing of the protease B precursor Prb1p%2C localizes to ER in lumenal orientation%3B homolog of mammalian PIG-X

Sequence (the first 754 bases correspond to coordinates 25109..25862 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]