Part:BBa_K277030

3L.3_23.B1.04

3L.3_23.B1.04 is 753 bases long and is cloned into the pGem-T vector.

3L.3_23.B1.04 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B1.04 is a constituent of 3L.3_23.B1 (along with 3L.3_23.B1.01, 3L.3_23.B1.02, 3L.3_23.B1.03, 3L.3_23.B1.05, 3L.3_23.B1.06, 3L.3_23.B1.07, 3L.3_23.B1.08, 3L.3_23.B1.09, 3L.3_23.B1.10, 3L.3_23.B1.11, 3L.3_23.B1.12, 3L.3_23.B1.13, and 3L.3_23.B1.14.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

forward_primer YCL054W_tagf2v1 (446..473)

gene YCL054W (258..+2783) AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation%3B methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52%2C U2921%3B suppressor of PAB1 mutants

Sequence (the first 753 bases correspond to coordinates 21395..22147 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 447
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]