Part:BBa_K277021
3L.3_23.A2.09
3L.3_23.A2.09 is 839 bases long and is cloned into the pGem-T vector.
3L.3_23.A2.09 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.09 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.)
This part contains at least part of the following features (positions offset from first base of sequence):
kind and name offset notes
reverse_primer YCL057W_tagr1v1 (473..500)
forward_primer YCL057W_tagf1v1 (278..305)
mutation_affecting_coding_sequence YCL057W_re_remove_AlwNI (594..605) removal of AlwNI
gene YCL057W (153..+2291) Zinc metalloendopeptidase%2C found in the cytoplasm and intermembrane space of mitochondria%3B with Cym1p%2C involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins
Sequence (the first 839 bases correspond to coordinates 14751..15589 in synthetic chromosome yeast_chr3_3_23)
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 243
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 486
Illegal SpeI site found at 243 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 712
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 243
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 243
- 1000COMPATIBLE WITH RFC[1000]
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