Other

Part:BBa_K277019

Designed by: James DiCarlo   Group: iGEM09_Johns_Hopkins-BAG   (2009-10-20)


3L.3_23.A2.07

3L.3_23.A2.07 is 815 bases long and is cloned into the pGem-T vector.

3L.3_23.A2.07 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.07 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.04, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

gene YCL059C (-715..235) Essential nucleolar protein required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit

gene YCL058W-A (440..781) Protein of unknown function%3B identified by homology to Ashbya gossypii

mutation_affecting_coding_sequence YCL058C_stop_recode_overlap_from_YCL058W-A (781..781) Overlaps stop codon change in YCL058W-A

gene YCL058C (379..+837) Protein of unknown function%2C required for survival upon exposure to K1 killer toxin%3B involved in ion homeostasis

Sequence (the first 815 bases correspond to coordinates 13280..14094 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 15
    Illegal XbaI site found at 42
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 15
    Illegal XbaI site found at 42
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 15
    Illegal XbaI site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]


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