Part:BBa_K277016
3L.3_23.A2.04
3L.3_23.A2.04 is 743 bases long and is cloned into the pGem-T vector.
3L.3_23.A2.04 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A2.04 is a constituent of 3L.3_23.A2 (along with 3L.3_23.A2.01, 3L.3_23.A2.02, 3L.3_23.A2.03, 3L.3_23.A2.05, 3L.3_23.A2.06, 3L.3_23.A2.07, 3L.3_23.A2.08, 3L.3_23.A2.09, 3L.3_23.A2.10, 3L.3_23.A2.11, 3L.3_23.A2.12, 3L.3_23.A2.13, and 3L.3_23.A2.14.)
This part contains at least part of the following features (positions offset from first base of sequence):
kind and name offset notes
reverse_primer YCL061C_tagr2v1 (607..634)
reverse_primer YCL061C_tagr1v1 (400..427)
gene YCL061C (-2120..+1170) S-phase checkpoint protein found at replication forks%2C required for DNA replication%3B also required for Rad53p activation during DNA replication stress%2C where it forms a replication-pausing complex with Tof1p and is phosphorylated by Mec1p%3B protein involved in replication checkpoint
forward_primer YCL061C_tagf1v1 (7..34)
forward_primer YCL061C_tagf2v1 (136..163)
Sequence (the first 743 bases correspond to coordinates 11072..11814 in synthetic chromosome yeast_chr3_3_23)
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 484
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 484
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 484
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 484
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 694
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