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Part:BBa_K277009

Designed by: James DiCarlo   Group: iGEM09_Johns_Hopkins-BAG   (2009-10-20)


3L.3_23.A1.09

3L.3_23.A1.09 is 744 bases long and is cloned into the pGem-T vector.

3L.3_23.A1.09 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.09 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.02, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

ARS ARS320 (-345..462) Autonomously Replicating Sequence on Chromosome III

forward_primer YCL064C_tagf1v1 (94..121)

reverse_primer YCL064C_tagr1v1 (337..364)

gene YCL064C (-14..+1068) Catabolic L-serine (L-threonine) deaminase%2C catalyzes the degradation of both L-serine and L-threonine%3B required to use serine or threonine as the sole nitrogen source%2C transcriptionally induced by serine and threonine

Sequence (the first 744 bases correspond to coordinates 5880..6623 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 462
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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