Part:BBa_K277002

3L.3_23.A1.02

3L.3_23.A1.02 is 731 bases long and is cloned into the pGem-T vector.

3L.3_23.A1.02 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.A1.02 is a constituent of 3L.3_23.A1 (along with 3L.3_23.A1.01, 3L.3_23.A1.03, 3L.3_23.A1.04, 3L.3_23.A1.05, 3L.3_23.A1.06, 3L.3_23.A1.07, 3L.3_23.A1.08, 3L.3_23.A1.09, 3L.3_23.A1.10, 3L.3_23.A1.11, and 3L.3_23.A1.12.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

gene YCL073C (-320..+1527) Protein of unconfirmed function%3B displays a topology characteristic of the Major Facilitators Superfamily of membrane proteins%3B coding sequence 98% identical to that of YKR106W

Sequence (the first 731 bases correspond to coordinates 734..1464 in synthetic chromosome yeast_chr3_3_23)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 172
  Illegal BsaI.rc site found at 219