Composite
JTPI

Part:BBa_K2768010

Designed by: Wenfei Yu   Group: iGEM18_USTC   (2018-10-02)


Positive feedback, expressing downstream gene.

We compose luxR-Terminator-lux pR-luxI part, and it has a positive feedback activated by AHL. Lux pR has a low expression level, but once be activated by LuxR-AHL compound, it's expression level would acutely rise, which lead to more expression of downstream gene and LuxI. We thought there would be a lower limits of concentration of AHL, but experiments shows that it can self-activate itself.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1705
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985


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