Composite

Part:BBa_K2765051

Designed by: Fa He   Group: iGEM18_BIT-China   (2018-10-08)


Complete roGFP2-Orp1 expression cassette started by the promoter TEF1

This part consists of the promoter TEF1p , redox sensor roGFP2-Orp1 and the terminator CYC1t. The promoter TEF1p is an constitutive promoter from Saccharomyces cerevisiae CEN.PK2-1c（see the details in part：BBa_K2765045）. roGFP2-Orp1 is a fusion protein which can response to the redox status in the cell rapidly as well as sensitively(see the details in part:BBa_K2765043).

T--BIT-China--iGEM2018-PartsOutput-TEF1geneline.1.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1251
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 157
    Illegal SapI.rc site found at 1563


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Categories
Parameters
None