Coding

Part:BBa_K2765030

Designed by: Jun Li   Group: iGEM18_BIT-China   (2018-10-07)


1000bp Homologous arm+Ura(△yca1)

Introduction

This part consists of the upstream homologous arm and the downstream homologous arm of yca1 and ura marker .yca1 is the yeast endogenous gene，it can control the apoptosis of yeast cell .In order to improve the tolerance of yeast cells to high levels of ROS and construct a biological detection system to complete the function of antioxidant detection and screening, we knocked out the yca1 gene.as shown in Fig.1.


T--BIT-China--iGEM2018-Partsregulator-knockingoutyca1.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2416
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1825
    Illegal BsaI.rc site found at 277
    Illegal SapI.rc site found at 559
    Illegal SapI.rc site found at 580
    Illegal SapI.rc site found at 1672


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Categories
Parameters
None