Coding

Part:BBa_K2765021

Designed by: Jun Li   Group: iGEM18_BIT-China   (2018-09-15)


yno1

Usage

Yno1(also known as aim14，short for Altered Inheritance rate of Mitochondria or ygl160w) encodes a genuine NADPH oxidase (NOX enzymes), which is located in the endoplasmic reticulum (ER) and produces superoxide in a NADPH-dependent fashion. Experiments have shown that overexpress yno1 can increase the accumulation of intracellular ROS.

T--BIT-China--iGEM2018-Partsregulator-1.png

reference: https://www.yeastgenome.org/locus/S000003128

Biology

T--BIT-China--iGEM2018-Partsregulator-2.png


T--BIT-China--iGEM2018-Partsregulator-3.png


Overexpression yno1 gene can causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1569


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Categories
Parameters
None