Composite

Part:BBa_K2760032

Designed by: iGEM18_TecMonterrey_GDL   Group: iGEM18_TecMonterrey_GDL   (2018-10-12)


Signal peptide negative control characterization construct in L. rhamnosus

This construct is meant to be used as a negative control in characterizations of the signal peptides SP1-Nsp4 and SP2-Nsp2 in L. rhamnosus, it lacks signal peptides but is composed from the same elements as the previous constructs.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 38
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 724
    Illegal AgeI site found at 836
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None