Composite

Part:BBa_K2760030

Designed by: iGEM18_TecMonterrey_GDL   Group: iGEM18_TecMonterrey_GDL   (2018-10-12)


SP1-Nsp4 characterization construct L. rhamnosus

This is part of a series of constructs were codon optimized for L. rhamnosus as a way to test whether this signal peptides for secretion that work on E. coli, would work on L. rhamnosus, via the Sec secretion pathway and SRP pathway.

This construct is meant to be used in characterizations of the signal peptide SP1-Nsp4 in L. rhamnosus, which would be excreted into the media by the Sec-dependent (Sec) secretion pathway, the general secretion E. coli route. When this signal peptides are linked to the N-terminus of recombinant proteins, they are critical for translocation and secretion of proteins in E. coli [1].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 38
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 202
    Illegal AgeI site found at 784
    Illegal AgeI site found at 896
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None