Reporter

Part:BBa_K2760024

Designed by: iGEM18_TecMonterrey_GDL   Group: iGEM18_TecMonterrey_GDL   (2018-10-11)


iLOV Optimized both for L. rhamnosus GG and E.coli

iLOV is a fluorecent protein, that has been widely used as a reporter for several expression vectors. It has been noted for its photostability and for its convenient size, as GFP has 400bp more than iLOV. The iLOV sequence was modified in order for it to be expressed in L. rhamnosus GG. We have also added a His-Tag at the end to facilitate purification with His-Tag columns. Another user could omit the His-Tag sequence if it is not needed, the exact position is indicated in sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 264
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 196


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Categories
Parameters
None