Composite

Part:BBa_K2760022:Design

Designed by: iGEM18_TecMonterrey_GDL   Group: iGEM18_TecMonterrey_GDL   (2018-10-10)


Positive Control for Characterization of Signal Peptides (DsbAss)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 379
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 311


Design Notes

Designed for E. coli


Source

This plasmid expression vector has a T7 promoter (BBa_I712074), it contains a medium strength T7 promoter (BBa_Z0262), DsbAss signal peptide (BBa_K2760001) , iLOV (BBa_K2760024) as the fluorescent reporter with a His-Tag (BBa_K2760012) attached to it; and finally a double terminator (BBa_B0015).

References