Composite

Part:BBa_K2760021:Design

Designed by: iGEM18_TecMonterrey_GDL   Group: iGEM18_TecMonterrey_GDL   (2018-10-10)


SP2-Nsp2 Characterization construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 382
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 314


Design Notes

The sequence is codon optimized for E. coli BL21


Source

This plasmid expression vector has a T7 promoter (BBa_I712074), it contains a medium strength T7 RBS (BBa_Z0262), the signal peptide SP2-Nsp2 (BBa_K2760004), iLOV (BBa_K2760024) as the reporter protein with a His-Tag (BBa_K2760012) attached to it; finally a double terminator (BBa_B0015).

References

[1] Han, S., Machhi, S., Berge, M., Xi, G., Linke, T., & Schoner, R. (2017). Novel signal peptides improve the secretion of recombinant Staphylococcus aureus Alpha toxinH35L in Escherichia coli. AMB Express, 7, 93. http://doi.org/10.1186/s13568-017-0394-1