Composite

Part:BBa_K2760019:Design

Designed by: iGEM18_TecMonterrey_GDL   Group: iGEM18_TecMonterrey_GDL   (2018-10-10)


Characterization construct for SGP1-NSP4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 106
    Illegal AgeI site found at 382
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 314


Design Notes

  • For E. coli
  • Has different gene sequences into it


Source

It contains the sequences from T7 promoter (BBa_I712074), medium strenght T7 RBS (BBa_Z0262), SP1-NSP4 (BBa_K2760000) a compatible codon optimized (with L.rhamnosus) iLOV sequence (BBa_K2760024) , 6 His Tag (BBa_K2760012), Double Terminator (BBa_B0015).

References

[1] Han, S., Machhi, S., Berge, M., Xi, G., Linke, T., & Schoner, R. (2017). Novel signal peptides improve the secretion of recombinant Staphylococcus aureus Alpha toxinH35L in Escherichia coli. AMB Express, 7, 93. http://doi.org/10.1186/s13568-017-0394-1