Coding
SP1-NSP4

Part:BBa_K2760000

Designed by: Mariano del Toro   Group: iGEM18_TecMonterrey_GDL   (2018-10-03)


Signal peptide for the secretion of recombinant proteins (SP1-NSP4)

This signal peptide was taken from Han et al 2017 [1], the novel signal peptide 4 (NSP4) was selected because it improved the secretion of a desired target and even decreased induction time. The novel peptides designed in the study can be used to improve the secretion efficiency of other recombinant proteins , for E. coli expression platforms. This signal peptide is part of the Sec-dependent (Sec) secretion pathway, the general secretion E. coli route. Signal peptides linked to the N-terminus of recombinant proteins play a critical role in translocation and secretion [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 48
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None