Part:BBa_K2754001

RRvT with lacI promoter

This part encodes the red fluorescent protein RRvT.

The testing of the new improved biobrick was done in parallel to the existing BBa_J4450 biobrick. The first time we tested the biobrick we incubated the cell culture, with an OD of approximately 0.1, in a transparent 96 well plate. The parts were measured ca every five minutes for 30 hrs by a Tecan plate reader. When the absorbance reached 0.8 Isopropyl β-D-1-thiogalactopyranoside (IPTG) was added to see if this gave any effect on the fluorescence. A growth curve for absorbance and fluorescence were then made. These curves were used to determine how the fluorescence changed with time.

The following figure shows growth curves for RRvT and mRFP as absorbance against time.

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Figure 1: Growth curve of RRvT and mRFP1. After around 250 minutes, x mM IPTG was added.

Fluorescence plotted against time for the two biobricks are shown in the figure below:</p>
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Figure 2: Fluorescence for both biobricks plotted against time. The discontinuous points of the curve are due to a shut-down of the computer during measurements. x mM IPTG was added after around 250 minutes.

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Figure 3: Fluorescence for both biobricks with ph=5 without IPTG.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 233
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1468
  
• 1000
  COMPATIBLE WITH RFC[1000]