RRvT with lacI promoter
This part encodes the red fluorescent protein RRvT.
The testing of the new improved biobrick was done in parallel to the existing BBa_J4450 biobrick. The first time we tested the biobrick we incubated the cell culture, with an OD of approximately 0.1, in a transparent 96 well plate. The parts were measured ca every five minutes for 30 hrs by a Tecan plate reader. When the absorbance reached 0.8
Isopropyl β-D-1-thiogalactopyranoside (IPTG) was added to see if this gave any effect on the fluorescence.
A growth curve for absorbance and fluorescence were then made. These curves were used to determine how the fluorescence changed with time.
The following figure shows growth curves for RRvT and mRFP as absorbance against time.
Fluorescence plotted against time for the two biobricks are shown in the figure below:</p>
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BamHI site found at 233
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 1468
- 1000COMPATIBLE WITH RFC