Usage and Biology
This part is an improved part of part BBa_S03452, which is a part that contains a EGFP(Enhance Green Fluorescent Protein).
Albumin (ALB) signal peptide can be tagged to protein of interest and allow the protein of interest to be secreted extracellularly. This method has been proved by C. Attallah et al. We improved the part by point-mutating EGFP to changed the Van-Der-Waals force between identical EGFP molecules to decrease EGFP’s propensity to form dimers and by fusing the ALB signal peptide to our mutated EGFP, which becomes monomer EGFP(mEGFP) in order to make the mutated EGFP able to be secreted.
We ran electrophoresis to check if the length of the part is correct. Our insert contains ALB sequence and mEGFP sequence. We expect a product of 802bp (834bp along with a biobrick prefix and suffix). Gel photo is shown in the figure below.
reference: 1. A highly efficient modified human serum albumin signal peptide to secrete proteins in cells derived from different mammalian species. (2017, January 10). Retrieved from https://www.sciencedirect.com/science/article/pii/S1046592816305034
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BamHI site found at 780
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC