Plasmid

Part:BBa_K2751006

Designed by: Hao-Tung Liu   Group: iGEM18_NYMU-Taipei   (2018-10-10)


pET32a-FPFSkel1 CyPet LRP6BP3BP4

LRP6BP1 with RBS

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5437
    Illegal XbaI site found at 5167
    Illegal XbaI site found at 7152
    Illegal PstI site found at 1140
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5437
    Illegal NheI site found at 5212
    Illegal PstI site found at 1140
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5437
    Illegal BglII site found at 6699
    Illegal BamHI site found at 6930
    Illegal XhoI site found at 6512
    Illegal XhoI site found at 7659
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5437
    Illegal XbaI site found at 5167
    Illegal XbaI site found at 7152
    Illegal PstI site found at 1140
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5437
    Illegal XbaI site found at 5167
    Illegal XbaI site found at 7152
    Illegal PstI site found at 1140
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 3317
    Illegal NgoMIV site found at 3477
    Illegal NgoMIV site found at 5065
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1314
    Illegal BsaI site found at 6572
    Illegal BsaI.rc site found at 7582
    Illegal SapI.rc site found at 2396
    Illegal SapI site found at 6144


[edit]
Categories
Parameters
None