Plasmid

Part:BBa_K2751003

Designed by: Hao-Tung Liu   Group: iGEM18_NYMU-Taipei   (2018-10-09)


pET32a-FPFSkel1 YPet LRP6BP1BP2


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5167
    Illegal SpeI site found at 6747
    Illegal PstI site found at 1140
    Illegal PstI site found at 6229
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5212
    Illegal SpeI site found at 6747
    Illegal PstI site found at 1140
    Illegal PstI site found at 6229
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6482
    Illegal BamHI site found at 6925
    Illegal XhoI site found at 7654
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5167
    Illegal SpeI site found at 6747
    Illegal PstI site found at 1140
    Illegal PstI site found at 6229
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5167
    Illegal SpeI site found at 6747
    Illegal PstI site found at 1140
    Illegal PstI site found at 6229
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 3317
    Illegal NgoMIV site found at 3477
    Illegal NgoMIV site found at 5065
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1314
    Illegal BsaI.rc site found at 7577
    Illegal SapI.rc site found at 2396


[edit]
Categories
Parameters
None