Coding

Part:BBa_K2736109

Designed by: Lorenzo Pattiradjawane   Group: iGEM18_Utrecht   (2018-10-16)


CusS with Tar LBD

This part contains the coding sequence of the CusS protein with the aspartate sensing Ligand binding domain of the Tar receptor. This hybrid receptor activates CusR through phosphorylation in the presence of apsartate. CusR can then interact with several promoter elements of genes responsible for intracellular copper level maintenance.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 699
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 120
    Illegal SapI.rc site found at 937


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Categories
Parameters
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