Composite

Part:BBa_K2736108

Designed by: Lorenzo Pattiradjawane   Group: iGEM18_Utrecht   (2018-10-17)


Promoter Tar E309A

This part expresses a mutant aspartate receptor Tar. This part has a E309A mutation in the methylation helix. This mutation mimicks the mutated state of the receptor, reducing its affinity for aspartate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1323
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 152


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Parameters
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