Composite

Part:BBa_K2736103

Designed by: Lorenzo Pattiradjawane   Group: iGEM18_Utrecht   (2018-10-09)


promoter::cheZ::RLuc

Fusion of CheZ and renilla luciferase (Rluc) under control of the promoter part BBa_K608003. This protein emits light with a wavelength of 480 nm when coelenterazine is added to a final concentration of 5 μM. It can be used as light donor protein in BRET measurements. Alternatively, it can be used to rescue chemotaxis in E. coli strains.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1266


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Categories
Parameters
None