Composite

Part:BBa_K2736101

Designed by: Lorenzo Pattiradjawane   Group: iGEM18_Utrecht   (2018-10-09)


Promoter::eYFP::CheY

This composite part consists of BBa_K2736100 under the control of the constitutive promoter BBa_K608003. It expresses a eYFP::cheY fusion protein that emits light with a wavelength of 528 nm when excited with 480 nm. It can be used acceptor protein for BRET and FRET measurements in the chemotaxis pathway

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None