Plasmid
pYTK-AFA18

Part:BBa_K2732001

Designed by: Michael Barnhart   Group: iGEM18_USAFA   (2018-10-03)


Yeast Plasmid pYTK-AFA18

This plasmid consists of 8 individual parts described below it can be used in both E. coli and S. cerevisiae: 1) Assembly connector; ConLS - cloning location EcoR1 and Xba1 cut sites 2) Promoter; pTDH3; yeast promoter 3) Coding Sequence; mRUBY2; reporter 4) Terminator; tTDH1 - transcriptional terminator 5) Assembly connector; ConR1 - cloning site containing Spe1 and Pst1 6) S. cerevisiae marker; LEU2 (contains promoter, CDS, and terminator) 7) S. cerevisiae origin; CEN6/ARS4 8) E. coli marker and origin; AmpR-ColE1

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal XbaI site found at 15
    Illegal SpeI site found at 1987
    Illegal PstI site found at 1996
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal SpeI site found at 1987
    Illegal PstI site found at 1996
    Illegal NotI site found at 4457
    Illegal NotI site found at 6315
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal BglII site found at 855
    Illegal BamHI site found at 1578
    Illegal XhoI site found at 1593
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal XbaI site found at 15
    Illegal SpeI site found at 1987
    Illegal PstI site found at 1996
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal XbaI site found at 15
    Illegal SpeI site found at 1987
    Illegal PstI site found at 1996
    Illegal AgeI site found at 2909
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 4098


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Categories
Parameters
None